Green synthesis offers an environmentally sustainable alternative to
conventional chemical methods for the production of silver nanoparticles. In the
present study, an aqueous leaf extract of Ocimum sanctum was employed as a
biological reducing and stabilizing agent for the synthesis of silver nanoparticles.
The study was conducted in the Department of Botany, CCR College,
Muzaffarnagar, in collaboration with BIO-COSMOS Lab, Meerut, during
2025–2026. The synthesized nanoparticles were characterized using UV–visible
spectroscopy and agarose gel electrophoresis. The appearance of a characteristic
surface plasmon resonance peak near 420 nm was used to confirm nanoparticle
formation, while agarose gel electrophoresis was used to investigate
nanoparticle–DNA interactions and their potential effects on DNA integrity. The
findings are expected to contribute to a better understanding of the role of O.
sanctum phytochemicals in the bioreduction and stabilization of silver nanoparticles
and to support the development of safer, plant-mediated nanomaterials.
Green synthesis, silver nanoparticles, Ocimum sanctum, plant-mediated synthesis, nanoparticle characterization