spectroscopy (UV-VIS Spectrophotometer
Systronics Model 117) was the basic techniques and confirmed by peaks in the
UV-Visible spectrum corresponding to surface plasmon resonance (SPR) bands in
400–460 nm depending on concentration of leaf extracts, pH, temperature, time of
reaction of synthesis. Azadirechta indica derived biogenic AgNps. The green and
safe synthesis was also justified through noticeable color change of solution from
light yellow to brown on adding the extracts due to SPR peaks. These changes in
solution confirm the reduction of ions and formation of AgNPs in leaf extract. The
compounds present in the Azadirechta indica leaf extract acted both as reducing and
capping agent. Comprehensive characterization confirmed that Neem-mediated
AgNPs are predominantly spherical, crystalline, and well-stabilized by
phytochemicals from the plant extract. Agarose gel electrophoresis analysis
revealed that the synthesized AgNPs interact with DNA, inducing strand-breaking
activity as evidenced by the conversion of supercoiled DNA to open circular forms,
suggesting potential biological applications.
Biogenic silver nanoparticles (AgNPs), Azadirechta indica (Neem), UV-Visible absorption spectroscopy